Proteinuttrykk refererer til syntesen av proteiner within cells using information contained in the DNA or gene.
Proteiner are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study protein function in order to understand cellular processes.
This is currently studied based on using fluorescent proteiner as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for proteiner som er forbigående eller flyktige i naturen.
Forskerne har rapportert en ny teknikk på preprint-server 30. juli 2020 som kan overvinne denne begrensningen.
The new study describes the use a fluorescent biosensor that enables real-time detection of protein expression in vivo which has implications for understanding the spatiotemporal expression of proteiner within the living organism. This sensor is based on a dim green fluorescent protein in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a protein tag and enables detection of protein expression within seconds in live cells.
Dette biosensor will be useful to study biological processes in real-time where proteiner are either transiently expressed and/or to understand the expression and interactions of proteiner from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Referanse:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. Genetisk kodet fluorescerende biosensor for rask påvisning av proteinuttrykk. Fortrykk: bioRxiv 2020.07.30.229633; GJØR JEG: https://doi.org/10.1101/2020.07.30.229633
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